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KMID : 0357419950250010023
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Korean journal of Virology
1995 Volume.25 No. 1 p.23 ~ p.30
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Small, Round-Structured Virus in Seoul: Determination of its Importance and Direct Sequence of a Genome
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Abstract
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A large number of nonculturable small round-structured viruses (SRSVs) have been implicated in epidemic. acute gastroenteritis (AG) in humans since the first detection of the Norwalk virus (NV) by immune electron microscopy in 1972. The SRSVs are
termed
after the locations in which they were first found and, like caliciviruses, contain a single stranded RNA. Recently, outbreaks of AG due to the unknown SRSV (termed as Seoul-SRSV) occurred in seoul. A single hybridization band was observed with
Chiba-SRSV (a strain implicated in an AG outbreak in Chiba, Japan, in 1987). Diarrheal stools of sporadic cases also yielded the same SRSV, emphasizing the need for a systematic prevalence study of the SRSV in Korea. The objectives of this study,
therefore, were to investigate the proportion of AG of unknown etiology that were associated with the Seoul-SRSVs among Korean children and to determine their nucleotide/amino acid sequences for comparison with that of the NV, Chiba-SRSV, and
feline
calicivirus (FC). A random sample of specimens with and without an identifiable enteric virus was screened for the Seoul-SRSV by RT-PCR and Southern blotting with the Chiba-SRSV primers. Overall, 23(30%) of 77 cases and 1 (6%) of 16 controls
excreted
the SRSV (p<0.05), the second most frequently demonstrated viral agent in this study. Our results suggest that the SRSVs are prevalent in Korea. Since Seoul-SRSV represents only a single serotype of SRSVs, the aggregate pathogenic potential of
these
agents of pediatric diarrhea in Korea may be considerable. Partial sequencing of two Seoul-SRSVs showed that the fragment analysed contains 353bp and a putative open reading frame (ORF). Significantly deduced amino acid sequence homology was
found
by
comparison with other sequences of the Chilba-SRSV (100%), NV(70.1%), and FC(13.7%), The Seoul-SRSV may be regarded as a possible candidate for a member of Caliviridae, as proposed for the NV. The application of similar methods to the entire
genome
will
allow specific diagnostic assays and vaccines to be developed against the AG.
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KEYWORD
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